Deletion sequences are the most characteristic impurity of stepwise synthesis. Because they differ from the intended peptide by a single residue, they often elute close to it in reversed-phase HPLC, and mass spectrometry shows them as the full mass less that residue's mass.
Capping unreacted chains with acetic anhydride after each coupling turns potential deletion sequences into truncated, acetylated chains, which are easier to separate.
See also
Cite this page
Text
Cosmic Peptides. (2026, October 25). Deletion sequence: glossary entry. Cosmic Peptides Research Library. Retrieved October 26, 2026, from https://cosmicpeptides.com/research/glossary/deletion-sequence
BibTeX
@misc{cosmicpeptides_research_glossary_deletion_sequence,
author = {{Cosmic Peptides}},
title = {Deletion sequence: glossary entry},
howpublished = {Cosmic Peptides Research Library},
year = {2026},
month = oct,
url = {https://cosmicpeptides.com/research/glossary/deletion-sequence},
urldate = {2026-10-26},
note = {Last reviewed 2026-10-25}
} RIS
TY - ELEC AU - Cosmic Peptides TI - Deletion sequence: glossary entry T2 - Cosmic Peptides Research Library PB - Cosmic Peptides PY - 2026 DA - 2026/10/25 UR - https://cosmicpeptides.com/research/glossary/deletion-sequence Y2 - 2026/10/26 LA - en ER -
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