Peptides can associate through hydrophobic contacts and hydrogen bonding into oligomers, amorphous aggregates or ordered fibrils. Aggregation depends on sequence, concentration, pH relative to the isoelectric point, ionic strength, temperature and agitation.
Aggregates may be invisible yet change how a peptide behaves in an assay. They are studied with techniques such as size-exclusion chromatography, light scattering and dye-binding assays.
See also
For in vitro laboratory research only. Not for human or veterinary use. This library describes chemistry, analytical methods and published laboratory research; it gives no guidance on use in people or animals.